Protein Expression Services
CHO/HEK293 suspension culture with human-like post-translational modification, steadily producing milligram-to-gram quantities of target protein.
The expression system closest to native conformation — folding and post-translational modification are completed correctly, suiting therapeutic proteins and complex eukaryotic targets.
The mammalian platform uses CHO/HEK293 hosts with full protein folding and post-translational modification capability, giving proteins that closely match native higher-organism proteins in structure, physicochemical properties and function.
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1,000+ projects a month, with proven methods for hard-to-express proteins
Accurate folding plus complex glycosylation gives function close to the native protein
CHO/HEK293 suspension culture, milligram to gram scale
Gene optimisation through purification, fully customised
Eight stages. Mammalian glycosylation, phosphorylation and disulfide formation closely mirror human machinery; outputs and acceptance criteria are in the specifications below.
Codons optimised for CHO/HEK293 hosts and the gene synthesised
Cloned into a high-efficiency vector, sequenced and prepared
30 mL transient trial verifying expression level
QC by A280, SDS-PAGE and more, with the expression report delivered
1 L suspension scale-up, milligram-to-gram output
1–2 affinity steps to 85–90% purity at >1 mg/mL
Certificate of analysis (CoA) and all raw data delivered
Purified protein shipped under cold chain throughout
Gene synthesis through purified delivery totals roughly 7–10 weeks — widely used for antibodies, vaccines, cytokines and other structurally complex proteins needing precise modification.
Focused on CHO and HEK293 suspension culture, with post-translational machinery closely mirroring human cells — accurate folding and complex glycosylation bring function close to the native protein. Hard-to-express targets have proven optimisation routes, with steady milligram-to-gram output.
Deliverables, acceptance criteria and timelines by experimental stage. The staging differs from the process above: the process shows how a project runs, while this table is the acceptance basis written into the contract.
| Stage | Scope | Deliverables | Standards | Timeline |
|---|---|---|---|---|
| Gene synthesis & cloning | Codon optimization and gene synthesis, cloning into a high-efficiency expression vector, plasmid sequencing and preparation | Sequencing report (on request) | Sequence verified | 2-3 weeks |
| Small-scale expression & purification (30 mL) | Transient transfection; QC analysis (A280, SDS-PAGE, etc.) | Expression report; expression plasmid or strain (on request) | / | 2-3 weeks |
| Scale-up expression & purification (1 L) | Scale-up protein expression, purification (1–2 affinity steps), QC analysis (A280, SDS-PAGE, etc.) | Purified protein sample with certificate of analysis (CoA) | Protein yield 0.1–1 mg, purity 85–90%, concentration >1 mg/mL | 3-4 weeks |
Share the intended application and what you already have in hand. A scientist — not a sales rep — will scope feasibility, suggest the right route and send a quote, usually within one business day.
