Protein Expression Services
The prokaryotic platform of choice — fast, low-cost and high-yield, and especially strong on small proteins and hard-to-express membrane and toxic proteins.
As a classic prokaryotic expression system, it offers short experimental timelines, low overall cost and high expression yields, making it suitable for rapid production of non-glycosylated proteins, antibody fragments and intracellular proteins.
The platform uses E. coli as the expression host in a streamlined, efficient workflow that supports rapid scale-up of target proteins. The system does not provide eukaryotic glycosylation, and some proteins may form inclusion bodies; denaturation and refolding can be offered as an alternative process.
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1,000+ projects a month, with proven methods for hard-to-express proteins
Multiple expression strains and tag designs with optimised induction conditions meet varied requirements
Expression reports in as little as 2 weeks and purified protein in as little as 3 weeks
Codon optimisation, recombinant expression and purification, all customised
Eight stages, with a choice of expression strains and tag designs plus induction optimisation; outputs and acceptance criteria are in the specifications below.
Codons optimised for the E. coli host and the gene synthesised
Cloned into a high-efficiency vector (pET series and similar), sequenced and prepared
Plasmid transformed into the host strain for small-volume induction trials
Pilot-sample components are analyzed by SDS-PAGE and an expression verification report is delivered
1 L scale-up, with refolding optimisation where required
1–2 affinity steps to 85–90% purity at >1 mg/mL
Certificate of analysis (CoA) delivered, with optional endotoxin removal
Purified protein shipped under cold chain throughout
Vector construction takes 2–3 weeks, expression verification with solubility analysis 1–2 weeks, and 1 L scale-up 2–3 weeks; the expression report can arrive in 2 weeks and purified protein in 3.
Deep experience in soluble E. coli expression, purification and refolding, backed by many strains and vectors — with a choice of expression hosts, tag designs and induction conditions, plus optional services such as endotoxin removal.
Deliverables, acceptance criteria and timelines by experimental stage. The staging differs from the process above: the process shows how a project runs, while this table is the acceptance basis written into the contract.
| Stage | Scope | Deliverables | Standards | Timeline |
|---|---|---|---|---|
| Expression vector construction | Codon optimisation and gene synthesis, cloning into a high-efficiency expression vector, plasmid sequencing and preparation | Sequencing report (on request) | Sequence verified | 2-3 weeks |
| Expression check & solubility analysis | Plasmid transformation, protein expression and QC analysis (A280, SDS-PAGE, etc.) | Expression report; expression plasmid or strain (on request) | / | 1-2 weeks |
| Scale-up expression & purification (1 L) | Scale-up protein expression, purification (1–2 affinity steps), QC analysis (A280, SDS-PAGE, etc.) | Purified protein sample with certificate of analysis (CoA) | Protein yield 0.1–1 mg, purity 85–90%, concentration >1 mg/mL | 2-3 weeks |
Target A is a metal-dependent transcriptional repressor that regulates gene expression through metal-induced conformational change. The full-length fragment was engineered by molecular cloning, codon-optimised, built into pET21b and expressed in Rosseta for recombinant production and purification.
Share the intended application and what you already have in hand. A scientist — not a sales rep — will scope feasibility, suggest the right route and send a quote, usually within one business day.
