
Jun 10, 20268 min read




Platform & Equipment
Why an early-discovery CRO built its own cryo-EM, and what it actually solves in antibody epitope mapping.

Methods
Coating concentration, blocking, dilution series, cutoff selection and EC50 fitting — a step-by-step walkthrough of a reproducible binding assay.

Cell Lines
From kill curves to monoclonal picking — a reusable set of selection parameters and scoring criteria.

Immunization Strategy
With OD450 = 0.2 as the cutoff, the usual VHH and IgG titer thresholds — and booster strategies when titers run low.
Share the intended application and what you already have in hand. A scientist — not a sales rep — will scope feasibility, suggest the right route and send a quote, usually within one business day.