Antibody Discovery Platform
Antigen-specific B cells are captured straight from the immunized animal's PBMCs, with natively paired heavy and light chain genes recovered by single-cell RT-PCR.
Antigen-specific B cells are captured directly from the immunized animal's PBMCs, and natively paired heavy/light chain genes are recovered by single-cell RT-PCR — short timelines, high throughput, many positive clones, high affinity, and maturation already completed in vivo.
Because the chains are natively paired and already affinity-matured in vivo, no in vitro re-pairing or maturation is needed — the fundamental difference between this platform and in vitro display routes.
Rabbit monoclonal, mouse monoclonal and anti-idiotypic monoclonal services can all take this route; we recommend one at kickoff based on the target and its downstream use.
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Four stages. The platform serves as a screening route for rabbit and mouse monoclonal and anti-idiotypic services, starting once immunization is complete and the titre is met.
Peripheral blood mononuclear cells are isolated from the immunized animal
Labelled antigen captures specific B cells, which are sorted one by one
Natively paired heavy and light chain variable regions are amplified
Candidates are expressed recombinantly and binding verified, delivering positive clones with sequences
The single B cell platform captures antigen-specific B cells directly from PBMCs and recovers natively paired antibody genes by single-cell RT-PCR — short timelines, high throughput, many positive clones, high affinity and maturation already done in vivo.
Share the intended application and what you already have in hand. A scientist — not a sales rep — will scope feasibility, suggest the right route and send a quote, usually within one business day.
