Antibody Discovery Platform
Multiple rounds of in vitro affinity panning enrich specific binders against many difficult drug targets, including self-antigens and highly toxic molecules, overcoming in-vivo immune tolerance.
An ultra-large antibody library is constructed in a prokaryotic system, then subjected to multiple rounds of in vitro affinity panning to enrich specific binders against any target, including self-antigens and highly toxic molecules, without immune-tolerance constraints.
Selection is completed entirely in vitro, with stringency precisely adjustable as needed, making it a primary route for targets that rarely yield antibodies through immunization.
Alpaca and shark nanobody preparation use it as their primary route; rabbit monoclonal, mouse monoclonal and anti-idiotypic services can select it; and affinity maturation uses it for multi-round stringency screening.
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Four stages. The platform is the primary screening route for alpaca and shark nanobodies, and also serves rabbit and mouse monoclonals plus stringency screening in affinity maturation.
An ultra-large antibody display library is built in the prokaryotic system
With the target immobilised, repeated in vitro panning rounds enrich specific binders
Positive clones are screened by ELISA, then sequenced and analysed
Candidates are expressed recombinantly with binding and function verified
In the nanobody services this platform's library and screening stage delivers the enriched library, accepted at ≥1×10⁹ CFU library size, ≥95% insertion rate and ≥20-fold positive-clone enrichment (see each service's specifications).
Because selection happens entirely in vitro, immune tolerance is not a limit — specific antibodies can be enriched against any target, self-antigens and highly toxic molecules included. Ultra-large libraries with repeated affinity panning make it the core platform for nanobody discovery and stringency screening in affinity maturation.
Share the intended application and what you already have in hand. A scientist — not a sales rep — will scope feasibility, suggest the right route and send a quote, usually within one business day.
