Animal Immunization Services
An ultra-short 35-day cycle to a serum titre of ≥1:100,000, run end to end at our own alpaca facility under standardized protocols.
SanjingBio provides alpaca and rabbit immunization services under standardized protocols and strict quality control, run end to end by specialists with 15+ years of experience so every batch yields a high-quality antibody repertoire.
Alpaca immunization links seamlessly into our VHH screening (phage or yeast display), recombinant expression and functional characterisation — an integrated immunize–screen–validate route that shortens timelines and raises the yield of highly specific nanobodies.
The service accommodates targets across oncology, infectious disease, neurodegeneration and other active fields.
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Immunization completed in 35 days
Serum titre ≥1:100,000
For new targets, with no prior immune background in the serum
A lower-cost option for budget-sensitive projects
Eight stages. You may supply the antigen; after immunization, sampling and PBMC isolation, the project can continue seamlessly into VHH screening and downstream validation.
Antigen format, dose and boost schedule set from the target's structure
Supplied by you or commissioned; recombinant antigen purity ≥85%
Purity, concentration and integrity are checked before immunization
Repeat immunizations at our own facility, completed in 35 days by the specialist team
Peripheral blood is collected to schedule, with complete sample and immunization-batch records
Peripheral blood mononuclear cells are isolated from the immunized alpaca for subsequent VHH library construction
Serum titre by ELISA, delivered at ≥1:100,000
Immunization records, the titre report and 100 mL of immunized-alpaca whole blood delivered
The immunization cycle is 35 days; commissioned antigen preparation adds 1–6 weeks.
Immunization follows standardized protocols under strict quality control, run throughout by specialists with 15+ years of experience so every batch yields a high-quality antibody repertoire. It links seamlessly into VHH screening, recombinant expression and functional characterisation.
Raw titre data from three customer projects: after the third and fourth boosts, serum keeps a clear binding signal beyond 1:100,000 dilution while pre-immune serum stays at background throughout.
OD450 against serum dilution (1:1k–1:1024k); the three traces are pre-immune, third boost and fourth boost.
| Serum dilution | Pre-immune | 3rd boost | 4th boost |
|---|---|---|---|
| 1k | 0.086 | 3.9545 | 4.1473 |
| 2k | 0.0659 | 4.0657 | 3.9664 |
| 4k | 0.0592 | 3.8896 | 3.7873 |
| 8k | 0.0564 | 3.4219 | 3.6851 |
| 16k | 0.0561 | 2.498 | 3.4293 |
| 32k | 0.0548 | 1.5899 | 2.4115 |
| 64k | 0.0566 | 0.9709 | 1.5152 |
| 128k | 0.0565 | 0.4127 | 0.721 |
| 256k | 0.0578 | 0.2594 | 0.3612 |
| 512k | 0.0576 | 0.1533 | 0.2049 |
| 1024k | 0.0568 | 0.1012 | 0.1308 |
| blank | 0.0652 | 0.0674 | 0.0691 |
OD450 against serum dilution (1:1k–1:1024k); the three traces are pre-immune, third boost and fourth boost.
| Serum dilution | Pre-immune | 3rd boost | 4th boost |
|---|---|---|---|
| 1k | 0.0842 | 2.7358 | 3.3720 |
| 2k | 0.0897 | 2.5690 | 3.1712 |
| 4k | 0.0615 | 2.5158 | 3.1117 |
| 8k | 0.0633 | 2.2884 | 2.8404 |
| 16k | 0.0582 | 1.8025 | 2.6356 |
| 32k | 0.0867 | 1.1451 | 2.3246 |
| 64k | 0.0621 | 0.6415 | 1.4652 |
| 128k | 0.0447 | 0.3517 | 0.8944 |
| 256k | 0.0631 | 0.2162 | 0.4919 |
| 512k | 0.0439 | 0.1400 | 0.2873 |
| 1024k | 0.0557 | 0.1105 | 0.1879 |
| blank | 0.0624 | 0.0771 | 0.0831 |
OD450 against serum dilution (1:1k–1:1024k); the three traces are pre-immune, third boost and fourth boost.
| Serum dilution | Pre-immune | 3rd boost | 4th boost |
|---|---|---|---|
| 1k | 0.0567 | 3.9816 | 4.0347 |
| 2k | 0.0505 | 3.8400 | 3.9044 |
| 4k | 0.0625 | 3.6569 | 3.8197 |
| 8k | 0.0561 | 3.2971 | 3.5093 |
| 16k | 0.0827 | 2.7963 | 3.2288 |
| 32k | 0.0705 | 2.2454 | 2.5249 |
| 64k | 0.0598 | 1.8402 | 2.1806 |
| 128k | 0.0524 | 1.1205 | 1.2895 |
| 256k | 0.0526 | 0.7284 | 0.8864 |
| 512k | 0.0518 | 0.3460 | 0.2648 |
| 1024k | 0.0511 | 0.1889 | 0.2391 |
| blank | 0.0504 | 0.0518 | 0.0686 |
Share the intended application and what you already have in hand. A scientist — not a sales rep — will scope feasibility, suggest the right route and send a quote, usually within one business day.
