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ManualCOAMSDS
Guanylyl cyclase C (GUCY2C, heat-stable enterotoxin receptor), a type I transmembrane guanylate cyclase, transduces signals via cGMP generation. The GUCY2C paracrine axis, comprising GUCY2C and its ligands guanylin/uroguanylin, protects intestinal mucosa from mutagenic, chemical and inflammatory damage. Murine GUCY2C-targeted CAR-T cells specifically recognize and eliminate GUCY2C-positive human colorectal cancer cells. This work establishes a human GUCY2C-specific CAR-T therapeutic strategy for metastatic colorectal cancer with GUCY2C expression.
| Synonyms | GUCY2C, GUC2C, STAR, STA receptor, hSTAR, GC-C |
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| Protein Construction ＆ Source | •Recombinant Human GUCY2C (Uniprot: P25092-1) Protein is expressed from HEK293 cells with hFc(IgG1) tag at the C-terminus. It contains AA Ser 24 - Gln 430.•The protein has a predicted MW of 72.1 kDa. The protein migrates as 85-120 kDa when calibrated against under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin | < 0.1 EU per ug as determined by the LAL method. |
| Purity | > 90 % as determined by SDS-PAGE. |
| Concentration ＆ Formulation | •3.96 mg/mL•Filtered through a 0.22 μm filter, stored in 25 mM Sodium Citrate, 1x PBS, pH 7.4. |
| Transportation ＆ Storage | This product is shipped under low-temperature conditions. Avoid repeated freeze-thaw cycles during handling.•Protein solution can be stored at 2-8°C for 2-7 days.•Samples are stable for 3 months from date of receipt at -20℃ /-80℃.•Recommend to aliquot the protein into smaller quantities for optimal storage. |
| Target background | Guanylyl cyclase C (GUCY2C, heat-stable enterotoxin receptor), a type I transmembrane guanylate cyclase, transduces signals via cGMP generation. The GUCY2C paracrine axis, comprising GUCY2C and its ligands guanylin/uroguanylin, protects intestinal mucosa from mutagenic, chemical and inflammatory damage. Murine GUCY2C-targeted CAR-T cells specifically recognize and eliminate GUCY2C-positive human colorectal cancer cells. This work establishes a human GUCY2C-specific CAR-T therapeutic strategy for metastatic colorectal cancer with GUCY2C expression. |
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